Cloning and Sequencing of Artemisia annua Squalene Synthase Gene and Its cDNA
- VernacularTitle:青蒿鲨烯合酶基因及其cDNA的克隆与测序
- Author:
Liling FENG
;
Qingping ZENG
- Publication Type:Journal Article
- Keywords:
ARTEMISIA ANNUA;
SQUALENE SYNTHASE GENE;
DNA, RECOMBINANT;
SEQUENCE ANALYSIS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To clone the squalene synthase gene of Artemisia annua L. for improving the quality and production of Artemisia annua L. by genetic engineering. 【Methods】 PCR amplification, RT-PCR amplification, ligation of the target fragment with a T-vector and sequence analysis of the interested gene were performed. 【Results】 An expected 3590 bp fragment was amplified by PCR and an expected 1257 bp fragment was amplified by RT-PCR. The two cloned fragments were identified by PCR and restriction enzyme digestion respectively. The preliminary sequence data indicated that the results obtained were similar to that from GenBank, and the difference was only found in several base pairs. 【Conclusion】 The squalene synthase gene and cDNA of Artemisia annua L. were successfully cloned and sequenced.
