RNA interference c-erbB-2 and c-raf-1 genes expression combined transfection in the Tca8113 cell lines
- VernacularTitle:RNAi沉默c-erbB-2、c-raf-1表达对人舌鳞癌Tca8113细胞的干预作用
- Author:
Ping JI
- Publication Type:Journal Article
- Keywords:
Tongue carcinoma;
RNA interfering;
Post transcriptional gene silencing(PTGS);
Combined transfection
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the proliferating inhibition and the mechanisms of dsRNA interference c-erbB-2 and c-raf-1 genes expression combined transfection in the human tongue carcinoma Tca8113 cell lines.Methods:There were 3 groups in our study,control group,normal control group and RNAi experimental group.At different time after liposome-mediated transfection,the cell proliferation,apoptosis,mRNA level,protein expressing level and cell cycle were observed by MTT,RT-PCR and electronic microscope.Results:According to the results of RNAi experimental group,the OD-value were 0.073( P
