Comparative study between protein profile of mice endometrium with blastocysts adhering and without blastocysts adhering
- VernacularTitle:小鼠胚泡黏附时已黏附侧与未黏附侧子宫内膜蛋白质组差异的研究
- Author:
Wei PAN
- Publication Type:Journal Article
- Keywords:
Proteome;
Two-dimensional gel electrophoresis;
Blastocyst;
Implantation
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Examining the difference in the protein profiling of endometrium between the blastocyst-adhering side and the side without blastocyst adhering,in order to study the asynchrony of blastocysts adhesion between two uteri.Methods:The endometrium with and without blastocyst adhering from the mice pregnant for 5 days were analyzed by two-dimensional gel electrophoresis and imaging analysis.Results:The number of matched protein spots between two gels was up to 82.5%.760 and 720 protein spots with a molecular mass between 14.0～75.4 and isoelectric points (pI)from 4～7 were catalogued from silver-stained gels of the blastocyst-adhering endometrium and the endometrium without blastocyst adhering respectively.At least 60 proteins were up-regulated or down-regulated by over two folds between two groups.Conclusion:the different expression of protein profile may be a cause underlying the the asynchrony of blastocysts adhesion between two uteri.
