Effects of environment in vivo and in vitro on the differentiation of bone mesenchymal stem cells into neural cells
- VernacularTitle:体内外环境对骨髓间充质干细胞向神经细胞分化的影响
- Author:
Benhui ZHUO
- Publication Type:Journal Article
- Keywords:
Mesenchymal Stem Cells;
Neural cells;
Differentiation
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of environment in vitro and in vivo on the differentiation of bone mesenchymal stem cells (MSCs) into neural cells.Methods:①MSCs were labeled with 4,6-diamidino-2-phenylindole (DAPI) and injected into the right brain of the rats(n=6),which had suffered from middle cerebral artery occlusion(MACO) a day before.Two weeks later,immunofluorescent staining was used to detect the expression of nestin,microtubule-associated protein 2 (MAP-2) and glial fibrillary acidic protein (GFAP).②In vitro,MSCs were cultured with modified neuronal medium(MNM)for 18 hours after being pretreated with 0.5umol/L all-trans retinoid acid (ATRA) for 24 hours.Immunocytochemistry was used to detect the expression of nestin,MAP-2 and GFAP.Results:①After being injected into rats for 2 weeks,MSCs survived in the ischemic areas and a few cells expressed nestin,MAP-2 and GFAP respectively.②In vitro,after ATRA and MNM treatment,the positive percentage of nestin and MAP-2 were (92.3?3.4)% and (89.6?3.3)% respectively,but no expression of GFAP was detected.Conclusion:The different ratio of MSCs differentiating into neural cells suggests that there is difference in the process and mechanism between environment in vitro and in vivo.
