Establishment of multidrug resistant hepatoma cell subline and its biological features
- VernacularTitle:人肝肿瘤耐药细胞亚系建立及生物学特性研究
- Author:
Shiqiao LUO
- Publication Type:Journal Article
- Keywords:
Liver neoplasma;
Multidrug resistance;
Cell line
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish multidrug resistant human hepatoma cell subline(HepG2/DOX) and study its biological features.Methods:Doxorubincin-resistant human hepatoma cell subline (HepG2/DOX) was induced by doxorubincin-gradually increasing concentration.To observe cells by light microscope and analyze number and structure of chromosomes of these tumor cells.Sensitivity of anticancer drugs was screened in HepG2,HepG2/DOX cells by MTT method.The immunohistochemistry method was used to measure MDR-1 gene product Pgp expression.Results:HepG2/DOX and HepG2 were different in shape,growth speed.The number and structure of their chromosomes were also different.The value of 50% inhibitory concentration(IC50,?g/ml) for DOX,CDDP,MMC,5-FU,and MTX in HepG2,HepG2/DOX cells was (0.070 and 1.161),(0.214 and 1.317),(0.162 and 0.498),(0.313 and 1.683),(0,007 and 0.217) respectively.MDR-l gene product Pgp170 expression was found either in HepG2/DOX or HepG2 cells.Pgp170 exprssion in HepG2/DOX was higher than in HepG2.Once HepG2/DOX had been cultured in DOX free RPMl1640 medium for 5 weeks,the IC50(?g/ml)for DOX in HepG2/DOX reduced to 0.684.Conclusion:HepG2/DOX has the characteristics of MDR.The drug-resistance was correlated with over expression of MDR-1 gene product Pgp 170 and slow growth.
