An experimental investigation on sodium hyaluronate in mid-Term cornea preservation
- VernacularTitle:透明质酸钠在角膜中期保存中的实验研究
- Author:
Juan YANG
- Publication Type:Journal Article
- Keywords:
Sodium hyaluronate;
Corneal endothelial cells;
Cornea preservation;
Eyebank technique
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the action of SH in mid-term cornea preservation.Methods:SH was added into mid-term preservation solution(MMK) to make the new storage media with SH of three different concentration (0.05%,0.03%,0.01%).The contrast group was MMK medium without SH.The corneal appearance,transparence and ultrastructure were observed,and trypan blue-alizarin red staining of CEC,SDH and G-6-P enzyme staining with histochemistry were made in different storage time.Results:The transparence of cornea and ultrastructure of CEC in all the experimental groups were better than that of the contrast.The activity degree of CEC was highest in group I,lowest in the contrast,and statistically significant among all the groups.Conclusion:SH plays an important role in protecting CEC in middle storage medium,and enhances the quality and prolongs the duration of the preserved corneas.The protective effect is determined by the consistence of SH.
