A Study on Pharmacokinetics Studies of Gentiopicroside in Beagle Dogs
- VernacularTitle:龙胆苦苷在Beagle犬体内药代动力学研究
- Author:
Yingju FENG
;
Fuzhao YANG
;
Wubao GUO
;
Ying GU
;
Wenji SUN
- Publication Type:Journal Article
- Keywords:
Gentiopicroside;
Beagle;
HPLC;
Pharmacokinetics
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for measuring serum content of gentiopicroside in Beagle dogs and to explore its internal pharmacokinetic features. Methods The rapid and sensitive HPLC method was adopted as external standard. After intravenous injection of gentiopicroside, serum samples of the Beagle were collected and measured on C18 reverse-phase chromatographic column after liquid -liquid extraction. Results The linear range of gentiopicroside was 0.1343～687.375 ?g?mL-1(r2=0.99993). The recovery was within 81.15 %～97.99 %. The limit of detection (LOD) was 0.1343 ?g?mL-1. Conclusion The pharmacokinetic process of gentiopicroside can be described as one-compartment model with intravenous injection , t1/2 = 1.1324 h, Ke = 0.5811 h-1, V= 0.5316 L?kg-1, CL = 0.3274 L?kg-1?h-1. This indicates that gentiopicroside can be quickly distributed and eliminated and is not liable to be accumulated in the body.
