Determination of Berberine Hydrochloride in Xiaozhi Granule by HPLC
- VernacularTitle:HPLC法测定消痔冲剂中盐酸小檗碱的含量
- Author:
Yi WU
- Publication Type:Journal Article
- Keywords:
Xiaozhi granule;
Berberine hydrochloride;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the content determination of berberine hydrochloride in Xiaozhi Granule. Methods HPLC was performed on ODS column, the mobile phase consisting of acetonitrile-0.5%acetic acid (20∶80)and detection wavelength at 346 nm. Results The linearity of berberine hydrochloride was good in the range of 3.84～38.4?g/mL (r=0.9998), and the average recovery was 100.52 %, RSD=l.08 %(n=5). Conclusion This method was simple, sensitive and accurate , and can be used for the quality control of Xiaozhi Granule.
