The gene expressions of nestin and stem cell factor in neurons after cerebral ischemia-reperfusion in rats
- VernacularTitle:脑缺血再灌注损伤后神经细胞巢蛋白和干细胞因子基因表达
- Author:
Qingli ZHENG
;
Weiwei GONG
;
Ling WANG
;
Chengyun SUN
;
Yunliang GUO
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Nestin;
Stem cell factor;
Rats
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the gene expressions of nestin and stem cell factor(SCF)in neurons after ischemia-reperfusion injury in rat brain. Methods Thirty-six adult female rats were subject to left middle cerebral artery occlusion for 1.5h and different hours of reperfusion. In site hybridization was used to examine the expression of nestin and SCF mRNA in the rats subjected to 2h, 6h, 12h, 24h, 2d, 3d, 7d, 14d of reperfusion and sham-operation group (n=4). Results (1) Nestin expression in cortex, striatum and extraventricular zone was weak in the sham-operation group, and increased markedly in the ischemic hemisphere compared with sham-operation group except of reperfusion 2h in cortex, 2h, 6h in striatum, 2h, 6h and 14d in extraventricular zone. (2)SCF expression in cortex, striatum and extraventricular zone was weak in the sham-operation group, and increased markedly in the ischemic hemisphere compared with sham-operation group except of reperfusion 2h, 6h, 12h in cortex, 2h, 6h in striatum, 2h and 14d in extraventricular zone. Conclusion It is suggested that SCF expression might enhance the proliferation of neural stem cells following ischemia-reperfusion in rats.
