THE EXPRESSION OF L-SELECTIN FACILITATES MURINE HEPATOMA CELL LINE HCa-F'S METASTASIS THROUGH LYMPH NODE
- VernacularTitle:L-选择蛋白可能介导小鼠肝癌HCa-F细胞经淋巴道转移的研究
- Author:
Min LIU
;
Lifen WANG
;
Jie ZHU
;
Peiman YANG
;
Jianin ZHANG
- Publication Type:Journal Article
- Keywords:
L-selectin;
Metastasis;
HCa-F cells;
615 mouse
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective L-selectin is an important adhesion molecule,which is the lymph cell's homing receptor specifically expressed on leukocytes.A non-lymphoma cell line-HCa-F has the high potential ability of metastasis to peripheral lymph nodes,whose character is similar to lymphoma's.We want to explore whether the HCa-F cell's metastasis mechanism is also similar to lymphoma's,that is to say,whether HCa-F cell can also express L-selectin and the expression of L-selectin bring about the lymph node metastasis character. Methods First,?-fetoprotein,CD3 and CD20's monoclonal antibodies were used to identify the origin of HCa-F cell;then we analysed whether the HCa-F cell can express L-selectin by means of flow cytometry analysis,RT-PCR,and Western blotting;finally,Stamper-Woodruff method was used to examine the L-selectin's function. Results Immunostaining showed that the HCa-F cell was ?-fetoprotein positive,CD3 and CD20 negative cell line,which suggests that it is a liver cell line.And results of flow cytometry,RT-PCR,and Western blotting showd that HCa-F cell can express L-selectin.Additionally,anti-L-selectin's antibody can inhibit the adhesion between HCa-F cells and frozen section of mouse lymph node.Conclusion L-selectin expressed on HCa-F cells is a functional molecule which can facilitate HCa-F cell's metastasis to lymph nodes.
