Sequence and analysis of human anti-HBs? light chain gene
- VernacularTitle:人抗HBs轻链基因的序列测定及序列分析
- Author:
Xiaofeng SHI
- Publication Type:Journal Article
- Keywords:
Human Ig light chain;
Sequencing analysis;
HBsAg
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain the clone and gene of light chain on human antibody against HBs.Methods:The phage displaying library was subjected to three rounds panning of “adherence-elution-amplification” with HBsAg coated in solid phase and help phage.The positive clone against HBsAg was characterized by ELISA assay and its P/N value was 2.415.The recombinant plasmid vectors from the positive clone was amplified by PCR with designed oligonucleotide primers of ? light chain.The ? chain PCR fragment was subcloned into plasmid pUC18 and sequenced.Results:The phage antibody against HBsAg was screened from a human immunoglouin phage displaying library.The pUC18-? recombinant plasmid was constructed and sequenced.The gene size of ? chain is 643bp including variable region and constant region.Conclusion:According to the sequenceing and ELISA assay,we suggest that ? chain is a human anti-HBs-? immunoglobuin.
