OVEREXPRESSION OF ZINC FINGER PROTEIN INHIBITS LPS-INDUCED EXPRESSION OF E-SELECTIN IN ENDOTHELIAL CELLS
- VernacularTitle:锌指蛋白基因对内毒素诱导选择素表达的影响
- Author:
Jianhong MI
;
Chuhong ZHU
;
Dajun YING
- Publication Type:Journal Article
- Keywords:
A20 gene;
E-selectin;
Lipopolysaccharide(LPS)
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Previous studies have demonstrated that LPS can induce endothelial cell activation and the expression of E-selectin. In this study, we examined whether A20 gene could inhibit the expression of E-selectin in endothelial cells induced by LPS. Methods With the help of DOTAP, endothelial cells were transfected with pCDNA3.1 EHA20. The postive cell clones were selected with G418.The stable transfection and expression of A20 in the endothelial cells were determined by immunofluorescence analysis. The E-selectin expression was checked by immunofluorescence, Western blot and in situ hybridization. Results Abundant A20 stable expression in endothelial cells transfected with pCDNA3.1 EHA20 was confirmed by immunofluorescence analysis. E-selectin expression increased in LPS-inducible endothelial cells. A20 gene inhibited 90% LPS-inducible E-selectin expression(P
