Peripheral blood CD~+_(34)-positive mononuclear cells participate in neovasculogenesis of human ovarian epithelial carcinoma
- VernacularTitle:卵巢上皮性癌患者外周血CD_(34)~(+)单核细胞参与肿瘤血管生成的研究
- Author:
Fenglian XU
;
Youji FENG
- Publication Type:Journal Article
- Keywords:
Endothelium, vascular;
Neovascularization, pathologic;
Ovarian neoplasms;
Carcinoma
- From:
Chinese Journal of Obstetrics and Gynecology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether endothelial cell (EC) progenitors (CD~+_(34)-positive mononuclear cells) participated in neovasculogenesis of ovarian epithelial carcinoma through in vitro and in vivo experiments, and to explore the mechanism of tumor neovasculogenesis. Methods CD~+_(34)-positive mononuclear cells were isolated from peripheral blood of ovarian epithelial carcinoma patients by means of magnetic beads coated with antibody to CD~+_(34), plated on culture dishes coated with human fibronectin in endothelium medium, and examined by using RT-PCR, fluorescence-activated cell sorting (FACS) and nitric oxide (NO) assay kit for the expression of EC lineage-markers. EC-like cells were labeled with DiI ex vivo, and injected into immunodeficiency mice model with transplanted hypodermic SKOV3 by caudal vein. After 4-6 weeks, the tumor was resected and examined by confocal microscopy, and immunohistochemistry. Results In vitro, CD~+_(34)-positive mononuclear cells differentiated into ECs. In animal models of SKOV3, EC progenitors (CD~+_(34)-positive mononuclear cells) incorporated into sites of neovasculogenesis in tumor, 4-6 weeks later DiI-labeled cells incorporated into capillaries and small arteries. Conclusions The neovasculogenesis in human ovarian epithelial carcinoma involves angiogenesis and vasculogenesis.
