The studies on the role of KAI1 gene in anti-metastasis of primary pancreatic cancer cells
- VernacularTitle:KAI1基因抗胰腺癌细胞转移的研究
- Author:
Jianhua XU
;
Xiaozhong GUO
;
Linan REN
- Publication Type:Journal Article
- Keywords:
Pancreatic cancer;
Metastasis;
KAI1 gene
- From:
Chinese Journal of Digestion
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of recombinant expressing plasmid pCMV-KAI1 on the proliferative ability of PANC Ⅰ and MiaPaCa Ⅱ pancreatic cancer cells, and to observe the suppress metastatic mechanism of KAI1 gene in the malignancy.Methods The plasmid pCMV-KAI1 was transfected into the human pancreatic cancer cell lines PANC Ⅰ and MiaPaCa Ⅱ with liposome. The proliferative ability of these two pancreatic cancer cell lines were analyzed with MTT and colony-forming test, the cycle pattern was assayed by flow cytometry.Results After KAI1 expressing plasmid tranfected into PANC Ⅰ and MiaPaCa Ⅱ, the cell growth rates of the two cell lines were reduced by 40.59% and 65.84% respectively compared with the control cells (P
