HPLC fingerprint analysis of Crocus sativus L. and comparative study on its different sources
- VernacularTitle:西红花药材高效液相色谱指纹图谱研究及不同产地药材谱比较
- Author:
Shuiyun ZENG
;
Sihai YANG
;
Changhua HUANG
- Publication Type:Journal Article
- Keywords:
Crocus sativus L.;
HPLC-FPS;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the method of fingerprint analysis of Crocus sativus L. and compare their HPLC-FPS of eight kinds of Crocus sativus L. from different sources. METHODS: HPLC with ZORBAX SB-C 18 column was used, the mobile phase was a linear gradient of methanol containing 1% acetic acid and water containing 1% acetic acid in 40min, the detection wavelength at 312nm. RESULTS: 12 marker peaks were separated. The methodological evaluation showed that the method had a good repeatability, and the marker peak area ratio of different samples were different. CONCLUSION: This method is simple and accurate with good reproducibility and can be used for a quality control for Crocus sativus L.
