Determination of fatty acids in Fructus Bruceae oil by precolumn derivation HPLC
- VernacularTitle:柱前衍生HPLC法测定鸦胆子油中的脂肪酸含量
- Author:
Yi DING
;
Xing TANG
- Publication Type:Journal Article
- Keywords:
Fructus Bruceae oil;
fatty acid;
precolumn derivation;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Object A method was developed for the determination of fatty acid in Fructus Bruceae oil by precolumn derivation HPLC Methods Fatty acids were derivatized with p bromophenacylbromide as derivative and 18 crown 6 as catalyst The method used C 8 Kromasil C 8 (250 mm?4 mm, 5 ?m) column and isocratic acetonitrile water eluent and the internal standard was heptadecanoic acid The detection wavelenghth was 254 nm Column temperature was fixed at room temperature, and the flow rate was 1 3 mL/min Results The standard curves of linoleic, palmitic, oleic, and steraric acid are linear within the range of 0 022—0 330, 0 014—0 213, 0 028—0 416, and 0 012—0 177 ?g, respectively The four fatty acid recoveries are 99 2%, 97 2%, 101 8%, 97 8%, and the RSD are 1 2%, 1 5%, 0 4%, 2 3%, individuslly Conclusion Five fatty acids are separated within 30 minutes during a single run The present method is reliable and relatively simple for the determination of fatty acid in Fruetus Bruceae oil
