Effects of nitric oxide synthase inhibitors on cerebral mitochondrial structure and function in fetal rats with intrauterine distress
- VernacularTitle:一氧化氮合酶抑制剂对宫内窘迫胎鼠脑组织线粒体结构及功能的影响
- Author:
Hua ZHANG
;
Zhenwei YAO
;
Weixin WU
- Publication Type:Journal Article
- Keywords:
Fetal distress;
Nitric-oxide synthase;
Mitochondria
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective lo evaluate the effects ot different types oi nitric oxide synthase inhibitors on cerebral mitochondrial structure and function in fetal rats with intrauterine distress. Methods Rats were divided into control group, acute ischemia group, treatment group 1 injection of [ N omega-nitro-L-arginine (L-NNA) 4 mg/kg into pregnant rats' abdomen before ischemia], reperfusion group, treatment group 2 [injection of L-NNA 4 mg/kg into pregnant rats' abdomen before ischemia followed by injection of aminoguanidine (AG) 500 mg/kg before operation]. Changes of mitochondrial structure were observed by transmission electron microscopy and expression of neuronal nitric oxide synthase (nNOS) mRNA (A) through RT-PCR. Inducible nitric oxide synthase ( iNOS) activity and mitochondrial Na+ K + -ATPase and cytochrome oxidase activity were measured. Results (1) The A of NOS(5 min,15 min) in acute ischemia group was higher than that of treatment group 1 ( P 0. 05). But the A of nNOS in two groups was higher than that in control group ( P 0. 05 ). Mitochondrial Rsv in reperfusion group was all smaller than those in treatment group 2. And mitochondrial Rsv in all the groups was smaller than that in control group( P
