Study on mitotic spindle and midbody extraction
10.3760/cma.j.issn.1673-4181.2014.02.003
- VernacularTitle:有丝分裂纺锤体及中间小体提取方法的研究
- Author:
Yan WU
;
Lina PAN
;
Changjun ZHU
;
Wei JIANG
;
Qimin ZHAN
;
Tong TONG
- Publication Type:Journal Article
- Keywords:
Mitotic spindle;
Midbody;
Cell cycle synchronization;
Mitosis
- From:
International Journal of Biomedical Engineering
2014;37(2):76-80,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective Mitotic spindle and midbody are both microtubule-based temporary structures during cell growth and play essential roles in mitosis.The purpose of this study was to establish a mature and efficient method to extract mitotic spindle and midbody.Methods Through the cell cycle synchronization method,mitotic spindle or midbody was made appear inside cells.Low permeability swelling and glycerol gradient centrifugation principles were then used to extract spindle and midbody.Results By Western Blot and immunofluorescence staining,the extracts were identified as mitotic spindle and midbody.Conclusions The successful extraction of mitotic spindle and midbody from synchronized Hela cells will provide foundation for identifying the proteins located in cell during mitosis,and be of great significance to the study of molecular regulation mechanisms of mitosis and tumorigenesis.
