Survivin variants' expression in gastric cancer cells and its relationshiop with proliferation and apoptosis
- VernacularTitle:生存素转录变异体在胃癌中的表达及其与细胞增殖、凋亡的关系
- Author:
Hua MENG
;
Yulei SUN
;
Dejian DAI
- Publication Type:Journal Article
- Keywords:
Survivin;
Real time reverse transcription-polymerase chain reaction;
Gastric cancer
- From:
Chinese Journal of Digestion
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of three survivin splicing variants in gastric cancer and normal gastric mucosa and to evaluate relationship among the survivin variants' expression and proliferation, apoptosis in gastric cancer. Methods Real time quantitative RT-PCR was used to analyze survivin variants expression in 77 paired tumors and normal gastric mucosa in frozen samples at the mRNA level. The cell proliferation and apoptosis were measured by Ki-67 immunoln's to chemical analysis and TUNEL method in paraffin-embedded block of same cases, respectively. Results The sarvivin splicing variants were remarkably up-regulated in gastic cancers compared with those in normal tissues (P
