Effect of retroviral HSVtk gene transfer on mice bladder tumor growth in vivo
- VernacularTitle:体内转导逆转录病毒HSVtk基因对鼠膀胱癌生长的影响
- Author:
Gang YE
- Publication Type:Journal Article
- Keywords:
Bladder cancer;
Retrovirus;
HSVtk;
GCV;
Gene therapy
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effectiveness of intratumoral and intraperitoneal injection with retrovirus HSVtk gene on mouse bladder cancer.Methods:Subcutaneous and intraperitoneal bladder tumor was respectively established with mouse cancer cell line(T739) in syngeneic mice.Direct intratumor injection of retroviral HSVtk into the subcutaneous tumor was performed.In peritoneum cavity tumor model,HSVtk gene was transferred by intraperitoneal injection of retrovirus vector carrying HSVtk gene.After GCV therapy the tumor volume and animal survival time were monitored.Results:Both animal models were successfully induced in all mice within one week following subcutaneous and intraperitoneal injection of T739 cells.Compared with control animals,the tumor growth in both models was significantly inhibited with HSVtk/GCV system,and also the animal survival time was prolonged with administration of GCV(P
