Induction of apoptosis in thyroid cells by iodide excess and its mechanism
- VernacularTitle:高碘诱导甲状腺细胞凋亡及其机制研究
- Author:
Dongfang LIU
- Publication Type:Journal Article
- Keywords:
Iodide excess;
Thyroid cell;
Apoptosis;
Apoptosis mechanism
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of excessive iodide in inducing apoptosis in thyroid cells and its mechanism.Methods:Normal primary thyroid cells were cultured in NaI of different concentrations with or without IL-1? and methimazole.The morphological changes in thyroid cells were observed by light microscopy and apotosis rate was determined by flow cytometry techique.In the meantime Fas,FasL,p53 level were determined by histochemistry.Results:Thyroid cells treated with iodide excess underwent the morphological changes and apoptosis. There was a gradually increased apotosis rate and Fas/FasL protein expression accompanying increase in iodide concentrations.No changes in p53 protein expression was shown. IL-1? enhanced the effect of iodide cytotoxicity,whereas methimazole inhibited the role of iodide inducing apotosis.Conclusion:These data indicate that excess molecular iodide induces apoptosis in thyroid cell through Fas/FasL channel, but independent of p53.
