Isolation,expansion and identification of fetal rat neural stem cell
- VernacularTitle:胚胎大鼠神经干细胞的分离培养及其鉴定
- Author:
Min ZHENG
- Publication Type:Journal Article
- Keywords:
Neural stem cell;
Self-renewing;
Multipotential proliferation;
Differentiation
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To culture neural stem cell (NSC) in vitro,observe its biological property and provide basis for further study on differentiation.Methods:NSC of rat fetus were isolated and cultured.Single-cell clone culture was used to confirm the character of self-renewing.Ability of multi-directional differentiation and specific antigen, nestin,were detected by immunocytochemistry (ICC).BrdU was used to show cell stage in division.Effect of combination of EGF,bFGF on proliferation of rat NSC were evaluated.Results:The cultured NSC showed multipotential of differentiation into neurons and glial cells,as well as the ability of self-renewing.Cells expressed nestin.The population of the NSC expanded quickly and the total number of the cell increased 2.45?10~4 folds after 15 times' passage.The property of NSC was stable after long culture and preservation.Combination of EGF and bFGF stimulated cell proliferation than used singly.Conclusion:The cultured NSC express nestin, have the ability of self-renewing and multi-directional differentiation.Combination of EGF and bFGF could promote the proliferation of rat NSC.
