A study on the effect of Genistein on migration of endothelial cells induced by human ovarian carcinoma cell line SKOV_3
- VernacularTitle:Genistein对人卵巢癌细胞系SKOV_3诱导内皮细胞迁移的作用及机制探讨
- Author:
Yu LI
- Publication Type:Journal Article
- Keywords:
Genistein;
Ovarian tumor;
Migration;
Endothelial cell
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Genistein on migration of human endothelial cells ECV-304 induced by human ovarian serous cystadenocarcinoma cell line SKOV_3,and to explore the mechanism of anti-angiogenesis of Genistein.Methods:Millicell chamber and coculture method were used to observe the influence on migration of ECV-304 induced by SKOV_3 cells or its conditioned media,and the effect of Genistein on migration of ECV-304 induced by SKOV_3 cells or its conditioned media without serum.The expression of angiogenesis associated protein VEGF,bFGF and TGF?-1 were determined using immunocytochemical method.Results:Either SKOV_3 cells or its conditioned media could induce the committed chemotactic migration of ECV-304.The chemotactic migrations of ECV-304 induced by SKOV_3 or its conditioned media were significantly inhibited by Genistein in a dose-dependent manner.20?mol/L Genistein could down-regulate the expression of VEGF and bFGF;up-regulate the expression of TGF?-1.Conclusion:Migrations of ECV-304 induced by human ovarian carcinoma cell line SKOV_3 or its conditioned media were apparently inhibited by Genistein.It suggests that this inhibitory effect of Genistein is completed by down-regulating the expression of vessel growth-promoting factor VEGF and bFGF,and up-regulating the expression of negative regulator TGF?-1.This may be one of the mechanisms of anti-angiogenesis of Genistein.
