An experimental study on cytokine and nitric oxide content in rabbit aqueous humor after alkali burn
- VernacularTitle:兔眼前段碱烧伤后房水细胞因子和一氧化氮在眼内炎症反应中的作用
- Author:
Li LI
- Publication Type:Journal Article
- Keywords:
Alkali burn;
Aqueous humor;
Endophthalmitis;
Tumor necrosis factor;
Nitric oxide
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship among inflammatory reaction and cytokine levels,nitric oxide(NO) content in aqueous humor after alkali burn.Methods:12 adult New Zealand rabbits were divided into two groups(6 rabbits each):normal control group and alkali burn experimental group.Aqueous humor samples were aspirated on the alkali burn 1,3,7,14 and 30 days,and the total number of white blood cells in the aqueous humor and their subset distribution as well as tumor necrosis factor-? (TNF-?) and NO~-_2/NO~-_3 were counted.The data were analyzed using analysis of variance of SPSS software.Results:There was a higher number of inflammatory cells and a higher level of TNF-? and NO~-_2/NO~-_3 in alkali burn eyes than that in the normal control eyes.The number of mononuclear phagocyte and the levels of TNF-?,the content of NO~-_2/NO~-_3 in aqueous humor were the highest in alkali burn group in 7～14 days.The change of TNF-?,NO~-_2/NO~-_3 and inflammatory reaction were basically similar,that is ,the more serious the reaction,the higher the levels of the content.Conclusion:The intraocular inflammation after alkali burn is closely related to the change in cytokine levels and NO content in aqueous humor.
