Application of chromatographic fingerprint of immature fruits of Terminalia chebula and its extracts
- VernacularTitle:西青果药材及提取物液相指纹图谱的应用研究
- Author:
Yuzhen YAN
;
Peishan XIE
;
Lifei SONG
;
Xiangxiang LIU
;
Pinghua LU
;
Xiaolin HUANG
- Publication Type:Journal Article
- Keywords:
immature fruits of Terminalia chebula;
in-process-quality-control;
chromatographic fingerprint
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: Terminalia chebula contained hydrolysable tannins up to about 35%. It was necessary to establish a chromatographic fingerprint to meet the quality control need effectively. METHODS: HPLC method was carried out with 3 kinds of the mobile phase , namely, A∶ 0.05mol?L -1 Phosphoric acid/ 0.05mol?L -1 Potassium dihydrogen phosphorate aqueous solution, B: methanol and C: Ethyl acetate, running in gradient mode based on the previous experiment. RESULTS: A marked peaks of HPLC fingerprint of the raw material, the extracts and its final product consisted of gallic acid, terchebulin, chebulamin, chebulagic acid and chebulinic acid. CONCLUSION: The fact has depicted that chromatographic fingerprint is a powerful tool for in-process-quality supervisory control and dynamic analysis of the active constituents during manufacture procedure of immature fruit products of Terminalia chebula.
