Determination of schisandrin, deoxy-schisandrin and ?-schisandrin in Jiangmeiling Capsules by HPLC
- VernacularTitle:RP-HPLC测定降酶灵胶囊中五味子醇甲、五味子甲素和五味子乙素的含量
- Author:
Zhengguo LI
;
Lizhuo YU
;
Aichen ZHANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Jiangmeiling Capsule;
schisandrin;
deoxyschisandrin;
?-schisandrin
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a HPLC method for the determination of schisandrin、 deoxy-schisandrin and ?-schisandrin in Jiangmeiling Capsule(extract of Fructus Schisandrae Chinensis). METHODS: The determination was performed by RP-HPLC on Kromasil TM C 18 column(200mm?4.6mm, 5?m) using methanol-H 2O (75∶25) as a mobile phase, flow rate at 1.0mL?min -1, detection wavelength at 224nm. RESULTS: The linear range of schisandrin was 0.02228～0.24508?g,r=0.9996. The average recovery was 101.87%, RSD=1.37% (n=5). The linear range of deoxyschisandrin was 0. 02188～0. 24068?g, r=0.9997. The average recovery was 99.75%, RSD=0.94% (n=5). The linear range of ?-schisandrin was 0.01975～0.2172?g, r=0.9996. The average recovery was 100.90%, RSD=0.99% (n=5). CONCLUSION: The method is convenient, sensitive and accurate. It can be a method for quality control in production of Jiangmeiling capsule.
