Determination of astragaloside Ⅳ in Qiluyishen Tabletes by HPLC-ELSD
- VernacularTitle:HPLC-ELSD测定芪鹿益肾片中黄芪甲苷的含量
- Author:
Yuerong CHEN
;
Lingyun MA
;
Shuangchen MA
- Publication Type:Journal Article
- Keywords:
Astragaloside Ⅳ;
Qiluyishen Tabletes;
HPLC-ELSD
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a method for the determination of Astragaloside Ⅳ in Oiluyishen Tabletes(Radix Astragali, Herba Pyrolae, Rhizoma Atractylodis Macrocephalae, Poria, Radix Codonopsis,etc.) by HPLC-ELSD. METHODS: Chromatography was performed on a 5?m HIQsilC 18 column(4.6mm?250mm) at 25℃. The mobile phase composition was acetonitrile-water(34∶66). The flow rate was at 1.0mL?min -1. An evaporation light-scattering detector (ELSD) was used as detector with gas flow rate of 2.6 mL?min -1 and drift tube temperature at 100℃. RESULTS: The linear range of Astragaloside Ⅳ was in the range of 2.288～ 22.88?g, r=0.999, the average recovery was 98.8% and RSD was 1.2%. CONCLUSION: This method is simple, feasible and reproducible. It can be used for the quality control of Oiluyishen Tabletes.
