Somatic embryogenesis and plantlet regeneration of Aconitum coreanum
- VernacularTitle:黄花乌头体细胞胚胎发生及其植株再生
- Author:
Rongmin YU
;
Xiuquan XU
;
Yu ZHAO
- Publication Type:Journal Article
- Keywords:
Aconitum coreanum (L?vl.) Rapaic s;
somatic embryogenesis;
plantlet regeneration
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop new resources of medicinal plan t of Aconitum coreanum (L?vl.) Rapaics by the method of plant cel l biotechnology. Methods The segments of stems, leaves, embr yonal axises, radicles, and cotyledon of A. coreanum were used a s the explants. The MS medium supplied with different auxin and cytokinin was e xamined. Results The calli were induced from explants on the MS medium supplied with 2, 4-D 1 mg/L and 6-BA 0.5 mg/L in the period of 30 d. And the calli were differentiated into green conus or globe embryo after se veral subcultures period on medium MS supplied with 2, 4-D 0.2 mg/L and 6-BA 0.5 mg/L. The adventitious buds differentiated could be developed from the so matic embryo callus into roots on medium MS supplied with IBA 0.5 mg/L, and the n the intact plantlets were obtained. Conclusion The plantle ts of A. coreanum can be obtained by the techniques of somatic embryogenesis and it is indicated that this may be a new way in developing new r esource and protecting of A. coreanum.
