Over-expression and purification of recombinant spike protein fragment of SARS coronavirus
- VernacularTitle:重组SARS-CoV刺突蛋白基因片段的原核表达及纯化
- Author:
Dan PU
- Publication Type:Journal Article
- Keywords:
SARS coronavirus;
Spike protein;
Expression;
Purification
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain the spike glycoprotein(S protein) of SARS coronavirus (SARS-Cov) for detection of the corresponding antibody and preparation of diagnostic kits.Methods:Genes encoding S protein were picked out to form a new gene and divided into two parts according to the prediction of antigenicty.They were obtained by nucleotide synthesis and were cloned into expression vector pET32a(+) respectively.Proteins then were induced by IPTG and purified through Ni-NTA.Results:Target proteins were successfully expressed and purified.Conclusion:The recombinant S proteins of SARS-CoV could be of great help in diagnosing the disease and investigating the virus further.
