Study on non-enzymatic separation and culture method of adipose-derived stem cells
10.3969/j.issn.1671-8348.2014.10.001
- VernacularTitle:脂肪源性干细胞无酶分离培养方法的研究
- Author:
Ping LIU
;
Chunmeng SHI
;
Lingli HU
;
Bo ZHANG
;
Zhengguo WANG
- Publication Type:Journal Article
- Keywords:
adipose derived stem cells;
separation method;
no-enzymatic;
collagenase digestion method;
cells,cultured;
adipose tissue
- From:
Chongqing Medicine
2014;(10):1153-1156,1160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a separation and culture method of human adipose-derived stem cells(ADSCs) suitable for the clinical application .Methods The non-enzymatic method and the collagenase digestion method were adopted to isolate and culture the cells from the human adipose tissue in the individuals with liposuction .The characteristics of isolated mesenchymal stem cells were comparatively analyzed .Results The required time in the non-enzymatic method was one third of that in the collagenase di-gestion method and the cellular morphology ,reproductive capacity ,immunophenotype and differentiation potential of the isolated cells were consistent to those isolated by the collagenase digestion method .Conclusion The no-enzymatic method may isolate and culture ADSCs from the adipose tissue in the individual with liposuction ,which is a safety and reliable isolation and culture method of human adipose tissue-derived stem cells suitable for clinical application .
