AN IMMUNOELECTRON MICROSCOPIC STUDY ON THE SYNAPTIC RELATIONSHIP BETWEEN DOPAMINERGIC AND GABAergic NEURONS IN THE BASOLATERALNUCLEUS OF THE RAT AMYGDALA
- VernacularTitle:大鼠杏仁体基底外侧核中DA能与GABA能神经元之间突触关系的免疫电镜研究
- Author:
Xiaoyun QIN
;
Juen HUANG
;
Naixiang LUO
;
Bojun WANG
;
Changwen JIANG
- Publication Type:Journal Article
- Keywords:
Dopaminergic axon terminal;
GABAergic neuron;
Synapse;
Amygdala;
Schizophrenia;
Electron microscopy;
Double immunostaining;
Rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between dopaminergic axon terminal and GABAergic neurons and explore the neuroanatomic mechanism of their effects in schizophrenia. Methods The co-location and the association of dopaminergic axon terminal and GABAergic neurons in the basolateral nucleus (BL) of rat amygdala were examined by using double labeling immunoelectron microscopic techniques. Dopaminergic axon terminal and GABAergic neurons were labeled with the antidopamine (anti-DA) and the anti-glutamic acid decarboxylase (anti-GAD) antibodies respectively. Results 43% of the DA-input synapses was observed to relate directly or indirectly to GAD-immunoreactive(IR) dendritic structures(DA/GAD), which includes single (38%), convergent (30%), serial (20%), and axoaxonic (12%) contact types.And 57% of the DA-input synapses was found to associate with unlabeled elements (DA/UE), which includes unlabeled perikarya (10%), dendrites (82%) and axons (8%). All of the synapses that show DA-IR terminals profiles were found to be symmetric (inhibitory) synapses.Conclusion These results suggest that the dopaminergic system in the BL of rat amygdala controls the mediations of the GABAergic interneurons via symmetric synapses. In addition, the dopaminergic axon terminal associates with the glutamatergic projection neurons and exerts influence on its activity.
