Production of antisense-vascular endotheilal growth factor (VEGF) 165 adenovirus and observation of the secreatory down-regulation by the infection of the antisense-VEGF165 adenovirus
- VernacularTitle:反义血管内皮生长因子腺病毒基因感染对类风湿关节炎滑膜细胞VEGF基因转录的影响
- Author:
Jianhua WANG
;
Huayang HUANG
;
Qingshui YIN
;
Jie WANG
;
Shengdong HUANG
- Publication Type:Journal Article
- Keywords:
Adenovirus;
Antisense gene;
Vascular endotheilal growth factor
- From:
Chinese Journal of Rheumatology
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the inhibition of vascular endotheilal growth factor (VEGF) gene transcribition and translation with antisense VEGF165 adenovirus vector transfection. Method To transfect the synovial cells with antisense VEGF165 adenovirus and detect the gene translation level with northern-blot method. Result The Northern-blot showed that: the transcription of VEGF gene were inhibited markedly after the antisense VEGF165 gene transfection for 3 days and the inhibition efficiency was obivious in 4th day. In the 5th day, the VEGF translation signal was hardly detected. Conclusion The antisense VEGF165 adenovirus gene transfection can inhibite not only the transcription but the translation of VEGF gene in synovial cells. Thus it can inhibit the secretion of VEGF in synovial cells with high efficiency.
