Determination of spinosin and ferulic acid in Suanzaoren Decoction by RP-HPLC
- VernacularTitle:RP-HPLC法测定酸枣仁汤中棘苷和阿魏酸的含量
- Author:
Yujuan LI
;
Kaishun BI
- Publication Type:Journal Article
- Keywords:
Suanzaoren Decoction (SZRD);
spinosin;
ferulic acid;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop an RP-HPLC method for determination of spinosin and ferulic acid in Suanzaoren Decoction (SZRD). Methods Hypersil C 18 column was used. The mobile phase consisted of acetonitrile-water with 1% glacial acetic acid (17∶83) for spinosin and (16∶84) for ferulic acid. Detection wavelengths were set at 334 and 320 nm, respectively. The internal standard was p-hydroxybenzaldehyde for ferulic acid. Results The linear ranges for spinosin and ferulic acid were 0.960—13.44 ?g/mL (r=0.999 8) and 2.04—27.2 ?g/mL (r=0.999 7), respectively. The recoveries for them were 98.4% and 98.8%, respectively. The RSD were 2.5% and 2.8%, respectively. Conclusion This method is simple, rapids and accurate, and can be used for the determination of spinosin and ferulic acid in SZRD.
