Protoplast fusion in Dendrobium candidum and Gynostemma pentaphyllum
- VernacularTitle:铁皮石斛与绞股蓝原生质体融合的初步研究
- Author:
Xiaoyong WEI
;
Min ZHANG
- Publication Type:Journal Article
- Keywords:
Dendrobium candidum Wall.ex Lindl.;
Gynostemma pentaphyllum (Thunb.) Makino;
protoplasts;
protoplast fusion;
cell division
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To explore the protoplast fusion of Dendrobium candidum Wall ex. Lindl. and Gynostemma pentaphyllum. (Thunb) Makino. Methods The two kinds of protoplasts were fused by PEG method, and then cultured in the modified liquid medium containing 2 mg/L BA and 1 mg/L NAA. Results High yield, viability, and pure mesophyll protoplasts were isolated from D. candidum and G. pentaphyllum. Conclusion The first cell division occurres within three days after fusion. And some of the cells are divided three times.