Pharmacokinetic studies of scutellarein and glucuronide scutellarin in rats

Yue LIU; Yong HUANG; Yongxi DONG; Yonglin WANG; Lin ZHENG

白话标题:野黄芩素及其葡萄糖醛酸结合物在大鼠体内的药动学研究
作者: Yue LIU ; Yong HUANG ; Yongxi DONG ; Yonglin WANG ; Lin ZHENG
出版类型:Journal Article
关键字: scutellarein; glucuronide scutellarin; rats; plasma concentration; UPLC-MS/MS; pharmaco-kinetics
来源: Chinese Pharmacological Bulletin 2014;(5):711-714,715
国家China
语言:Chinese
摘要: Aims To establish a UPLC-MS/MS meth-od for the determination of plasma concentration of scutellarein and its metabolite and to study their phar-macokinetics in rat plasma. Methods The analysis was achieved by BEH C18 column with a mobile phase composed of 0 . 1 % formic acid in acetonitrile and 0 . 1% aqueous formic acid using step gradient elution. A TQD tandem mass spectrometry equipped with electros-pray ionization source was used as detector and opera-ted by multiple reaction monitoring( MRM) positive ion mode. After intravenous injection of scutellarein, the concentrations of scutellarein and its major metabolite glucuronide scutellarin in rat plasma were determined at different time points. The pharmacokinetic parame-ters were calculated by DAS 2. 0 software. Results Good linearity was achieved for scutellarein, the ex-traction recovery was between 80 . 5 % to 90 . 5 %, the precisions and accuracy were good. The result showed the pharmacokinetic profiles of scutellarein and glucu-ronide scutellarin both fit to the two-compartment mod-el. Conclusion The above mentioned method is spe-cific, rapid, sensitive and suitable for the pharmacoki-netic studies of scutellarein and its metabolite.