Cloning and sequence analyzing of the recombinant plasmid affecting gene DNMT1 translation by RNA interfering
- VernacularTitle:DNMT1靶向RNA干扰重组体的构建及序列分析
- Author:
Daorong CHEN
- Publication Type:Journal Article
- Keywords:
DNMT1;
RNA interfering;
Cloning;
Sequence analysing
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone the recombinant plasmid affecting gene DNMT1 translation by RNA interfering and analyze the nucleic acid sequence of the recombinant for further searching new gene therapy method of tumor.Methods:Two DNA sequences containing small hairpin structure were designed and synthesized.The complement form was obtained by annealing and inserted into vector pTZU6+1,and the recombinant plasmid was transformed into Jm109 strain.Finally the plasmid identified by restriction enzyme was used for sequence analysis.Results:The recombinant was cloned and the aim sequence was obtained.Conclusion:Successful cloning of the recombinant helps to search new gene therapy method of tumor.
