Construction of a recombinant adenovirus vector containing HBX gene
- VernacularTitle:HBX基因重组腺病毒载体的构建
- Author:
Tao FENG
- Publication Type:Journal Article
- Keywords:
Gene;
HBX;
HBV;
Adenovirus vector;
Hepatitis B
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a recombinant adenovirus vector carrying HBX gene for investigation of the functions of HBX gene on the hepatocellular carcinoma.Methods:HBX gene was amplified from genomic DNA of HBV by polymerase chain reaction(PCR) technique, and subcloned to plasmid pHAHA to construct recombinant plasmid pHAHA-HBX.subcloned HAHA-HBX into a shuttle vector pAdTrack-CMV.After being identified by endonuclease,and subsequently cotransformed into E.coli.293 cells with an adenoviral backbone plasmid,e.g.pAdEasy-1.Results:HBX gene was cloned from HBV genome by PCR successfully,and proved by sequence determination.A vector producing cell line 293/HBX was established.An HBX gene integration was detected by PCR in the recombinant adenovirus vectors.Conclusion:A recombinant adenovirus vector containing HBX gene was successfully constructed.
