Construction and expression of the eukaryotic coexpression plasmid of Mycobacterium tuberculosis Ag85B and MPT64 gene
- VernacularTitle:结核分枝杆菌Ag85B与MPT64真核共表达质粒的构建及表达
- Author:
Shan JIANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Ag85B;
MPT;
Coexpression vector
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct an eukaryotic coexpression plasmid containing Mycobacterium tuberculosis Ag85B and MPT64 gene.Methods:Mycobacterium tuberculosis Ag85B and MPT64 gene were cloned into pBudCE4.1 to construct recombinant plasmid pBud85B-MPT.The recombinant plasmid was transfected into COS-7 cells,and its expression of Ag85B and MPT64 was assesed by RT-PCR.Results:Mycobacterium tuberculosis Ag85B and MPT64 were detected in transfected COS-7 cells.Conclusion:The recombinant plasmid pBud85B-MPT can express Ag85B and MPT simultaneously in COS-7 cells which provides the basis for further investigation of DNA vaccine against tuberculosis.
