Effects of sodium butyrate on the growth and apoptosis of human glioma cell line SHG-44
- VernacularTitle:丁酸钠诱导人脑胶质瘤细胞系SHG-44凋亡的初步探讨
- Author:
Yan ZHAN
- Publication Type:Journal Article
- Keywords:
Butyrate;
Glioma;
Apoptosis
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of sodium butyrate,the most important short chain fatty acids,on growth suppression and apoptosis of SHG-44 glioma cell line.Methods:Human glioma SHG-44 cells were treated with 2 mmol/L and 4 mmol/L sodium butyrate.The inhibition of cell growth was assessed by MTT spectropotometric analysis and immunohistochemisty methods.The apoptosis was observed by reverted microscope and transmission electron microscope.And mitochondrial transmembrane potential was detected by rhodamine 123 dyeing,which confirmed that SHG-44 cells suffered a mitochondrial path apoptosis.Results:Sodium butyrate treatment resulted in significant inhibition of cell growth,with proliferating cell nuclear antigen expression weakened.Furthermore,4 mmol/L sodium butyrate could induce the SHG-44 cells apoptosis.It was proved that cellular membrane shrunk,vacuoles existed in plasm,and apoptosis bodies occurred by morphologic observation.In addition,the △?m was decreased in apoptosis cells after 4 mmol/L sodium butyrate treatment.Conclusion:These results indicate,in malignant glioma SHG-44 cells,sodium butyrate could not only suppress cell growth,but also induce apoptosis by a mitochondrial path.
