Aspirin induces apoptosis on human myeloid leukemia cell line HL-60
- VernacularTitle:阿司匹林诱导HL-60细胞凋亡的实验研究
- Author:
Yun LUO
- Publication Type:Journal Article
- Keywords:
Aspirin;
Apoptosis;
HL-60
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of aspirin(ASA) on the apoptosis of human myeloid leukemia cell in vitro and its mechanisms.Methods:Inhibition rate was tested by MTT method.Apoptosis induced by ASA in HL-60 cells was investigated by applying light microscopy,TdT-mediated dUTP Nick-end Labeling(TUNEL) and flow cytometry analysis techniques.RT-PCR method were used to examine mRNA expression.Results:2.5～10.0mmol/L ASA can induce apoptosis and inhibit proliferation in HL-60 cells,and increase the sensitivity of HL-60 cells to Ara-c.ASA down-regulated the level of c-myc mRNA.Conclusion:A certain dosage of ASA can suppress the proliferation and induce apoptosis of acute leukemia cells in a dose dependent manner.One of the mechanisms of ASA inducing apoptosis may be related to down-regulated expression of c-myc mRNA.
