Detection of Antibiotic Resistant Genes in Salmonella enterica Serovar Typhimurium Isolated from Foodborne Patients in Seoul Using Multiplex-PCR.
- Author:
Young Hee OH
1
;
Mi Ok SONG
;
Moo Sang KIM
;
Seog Gee PARK
;
Young Ki LEE
Author Information
1. Seoul Metropolitan Government Health and Environment Research Institute, Korea. 2000jade@hanmail.net
- Publication Type:Original Article
- Keywords:
S. typhimurium;
Antibiotic resistance gene;
Multiplex-PCR;
Integron
- MeSH:
Ampicillin;
Anti-Bacterial Agents;
Bacteriophage Typing;
Chloramphenicol;
Diffusion;
Disease Outbreaks;
Drug Resistance, Microbial;
Humans;
Integrons;
Kanamycin;
R Factors;
Salmonella enterica*;
Salmonella*;
Seoul*;
Streptomycin;
Tetracycline;
Trimethoprim
- From:Journal of Bacteriology and Virology
2005;35(3):183-190
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The frequency of antibiotic resistance among Salmonella enterica serovar Typhimurium has increased due to the transfer of multiple resistance factors. We detected the 13 antibiotic resistance genes by multiplex-PCR and compared with the results of phage typing and antibiotic disk diffusion for 49 S. typhimurium isolated from food-poisoning outbreaks in Seoul from 1999 to 2002. Resistance genes for tetracycline, streptomycin, ampicillin, sulfonamide, amino-glycoside-modifying enzyme, chloramphenicol, kanamycin, and trimethoprim were detected in 67.3%, 57.1%, 26.5%, 8.1%, 8.1%, 5%, 2.0%, and 0% of isolates, respectively. Overall 28 isolates (57.1%) possessed two or more antibiotic resistance genes. Class 1 integron carrying multidrug resistace genes, ant(3")-IaB, blaPSE, qacE delta1/sul, and tet G were amplified especially in only DT104 isolates. Among the related resistance genes for same antibiotics, strA and strB for streptomycin resistance were simultaneously detected but tetA and tetB for tetracycline were sporadically detected. DT 104 isolates contained only aadA2 and tetG.
