Determination of Puerarin in Some Chinese Patent Medicines Containing Radix Purerariae by SPE- HPLC
- VernacularTitle:SPE-HPLC法测定中成药中葛根素的含量
- Author:
Jihui FANG
;
Binghui ZHU
;
Yuting ZOU
;
Jinxiong YU
- Publication Type:Journal Article
- Keywords:
Puerarin;
SPE;
Content determination;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To established a method for the determination of puerarin. Method The sample solutions were pretreated by solid phase extraction (SPE): the specimens were eluted and the cartridges saturated gradually by them in the SPE, but the strongly absorbed impurities were held by the cartridges all the same, and then the effluents were measured by HPLC. Results The linear range of puerarin was 0.205～ 2.464? g, r=0.9999. The average recovery of puerarin in Xintong Oral Liquid, Radix Purerariae and Ganmao Zhike Capsule were 99.5 % , 99.9 % and 98.5 % , RSD were 1.67 % , 1.45 % and 0.95 % (n=5) respectively. Conclusion The method is simple and accurate and can be used for the determination of puerarin in the Chinese patent medicines containing Radix Purerariae.
