Determination of Allantoin in Rhizoma Dioscoreae from Different Producing Areas by Reverse- phase HPLC
- VernacularTitle:反相高效液相色谱法测定不同产地山药中尿囊素的含量
- Author:
Haibo WANG
;
Baochang CAI
- Publication Type:Journal Article
- Keywords:
Rhizoma Dioscoreae;
Allantoin;
Content determination;
Reverse- phase HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of allantoin in Rhizoma Dioscoreae from different producing areas. Methods The RP- phase HPLC is applied. Separation and determination of allantoin was achieved by using an Allttima ODS C18 column(250 mm? 4.6 mm, 5? m) and a mobile phase of methanol- water (1 ∶ 39, V/V). The flow- rate was 0.8 mL/min, temperature at 30 ℃ and detection wavelength at 224 nm. Results A good linearity of allantoin was in the range of 0.24 ? g～ 2.40 ? g, r=0.9992; the average recovery rate was 103.1 % , RSD =2.0 % (n=6). Conclusion Rhizoma Dioscoreae from Henan Province is of the best quality and Rhizoma Dioscoreae from other provinces are only qualified for food.
