Extraction and Analysis of Genomic DNA from Different Parts of Herba Hedyotis Diffusae
- VernacularTitle:白花蛇舌草不同部位基因组DNA的提取分析
- Author:
Yongzhou YU
;
Miao ZHU
;
Zhiguo YU
- Publication Type:Journal Article
- Keywords:
Herba Hedyoeis Diffusae;
DNA;
Extraction and analysis
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assay the intact genomic DNA content of different parts from Herba Hedyoeis Diffusae and to observe its influence on the result of polymerase chain reaction (PCR) amplification. Methods The genomic DNA was analyzed by agarose gel electrophoresis and absorbance detection method and PCR amplification was carried out for further comparison. Results The content of intact genomic DNA in different parts was different as well as the DNA fingerprints of PCR amplification. Conclusion The obtaining of intact genomic DNA was the premise for further study.