cDNA microarray analysis of gene expression in acquired methotrexate-resistant of human choriocarcinoma
- VernacularTitle:甲氨蝶呤诱导人绒毛膜癌细胞耐药后耐药细胞基因表达谱的变化
- Author:
Yaxia CHEN
;
Xing XIE
;
Qi CHENG
- Publication Type:Journal Article
- Keywords:
Choriocarcinoma;
Methotrexate;
Drug-resistance, neoplasm;
Drug resistance, neoplasm;
Genes, MDR;
Oligonucleotide array sequence analysis
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the molecular components involved in acquired methotrexate-resistance of human choriocarcinoma. Methods Methotrexate-resistant cell line(JAR/MTX) was derived from JAR cell line by exposed to intervally and progressively increasing higher concentration of MTX. cDNA microarray analyses of JAR/MTX and its parental cell line JAR were performed. Results JAR/MTX was established after one year with stable resistance. Its resistant index to MTX was 7.3. Nine genes were differential expressed between JAR/MTX and JAR cells. INSR,SLC1A3,SAT,HBB,and FLJ12443 were underexpressed and HS1,TXNRD1,TAGLN2,and EEF2 were overexpressed in JAR/MTX cells. Conclusion The cDNA microarray system showed that several alterations of gene expression were present in acquired methotrexate-resistance of human choriocarcinoma.
