Nuclear Factor-kappa B Activation and Chemokine Genes Expression in HT-29 Intestinal Epithelial Cells in Response to Clostridium difficile Toxin A Stimulation.
- Author:
Jin Young LEE
1
;
Young Mee YOON
;
Hyun Cheol ROH
;
Jung Mogg KIM
Author Information
1. Department of Microbiology, Hanyang University College of Medicine, Korea. jungmogg@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
C. difficile toxin A;
Intestinal epithelial cells;
NF-kappaB
- MeSH:
Bacterial Toxins;
Chemokines;
Clostridium difficile*;
Clostridium*;
Epithelial Cells*;
Gene Expression;
HT29 Cells;
Humans;
I-kappa B Proteins;
Inflammation;
Interleukin-8;
NF-kappa B;
Retroviridae;
Transfection
- From:Journal of Bacteriology and Virology
2005;35(3):217-226
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Intestinal epithelial cells are known to up-regulate the expression of several chemokines in response to bacterial toxins. Since there has been little understanding on the cellular mechanisms of C. difficile toxin A-induced mucosal inflammation, we investigated whether nuclear factor-kappa B (NF-kappaB) could regulate chemokine gene expression in HT-29 intestinal epithelial cells stimulated with C. difficile toxin A. C. difficile toxin A rapidly increased signals of NF-kappaB composed with p65 and p50 subunits in HT-29 cells, whereas it decreased the signals of IkappaBalpha. Blocking the NF-kB activation by transfection with dominant negative I kappa B alpha-containing retrovirus attenuated the upregulated expression of IL-8, GRO-alpha, and MCP-1 induced by C. difficile toxin A. These results suggest that NF-kappaB is a major regulator of chemokine gene expression in C. difficile toxin A-stimulated intestinal epithelial cells.
