Cloning and expression of survivin gene in eukaryotic cells
- VernacularTitle:凋亡抑制因子survivin基因的克隆及在真核细胞中的表达
- Author:
Ge YAN
- Publication Type:Journal Article
- Keywords:
Survivin;
Green fluorescent protein;
Eukaryotic cell;
Expression
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone and express survivin gene in mammalian cells.Methods:Coding sequence of survivin gene was amplified from HepG 2 cell mRNA by RT-PCR and then cloned into eukaryotic vector pEGFP-C1.The vector with survivin gene was transfected into HepG 2 and SMMC-7721.The expression of survivin was detected by GFP and Western blot.Results:The recombinant plasmid pEGFP-C1-survivin was successfully constructed and expressed in HepG 2 and SMMC-7721.Conclusion:The result shows expression of the anti-apoptosis gene survivin in mammalian cells.
