Induction of TNF-alpha, IL-12, IL-10, MCP-1 and IL-8 in Human Peripheral Blood Monocytes in Response to the Cell Wall-Associated Triton X-100 Soluble Protein (TSP) Antigens of Mycobacterium tuberculosis.
- Author:
Ji Sook LEE
1
;
Tae Hyun PAIK
;
Yung Choon YOO
;
Junglim LEE
;
Chang Hwa SONG
;
Eun Kyung JO
;
Hwa Jung KIM
;
Jung Kyu PARK
Author Information
1. Department of Microbiology, College of Medicine, Konyang University, Nonsan, Chungnam, Korea. thpaik@konyang.ac.kr
- Publication Type:Original Article
- Keywords:
Mycobacterium tuberculosis;
TSP antigen;
IL-12
- MeSH:
Chemokine CCL2;
Cytokines;
Humans*;
Immunity, Innate;
Interleukin-10*;
Interleukin-12*;
Interleukin-8*;
Interleukins;
Monocytes*;
Mycobacterium bovis;
Mycobacterium tuberculosis*;
Mycobacterium*;
Neptune*;
Octoxynol*;
Phagocytes;
Tuberculin;
Tuberculosis;
Tumor Necrosis Factor-alpha*
- From:Journal of Bacteriology and Virology
2005;35(3):227-238
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Mycobacterium tuberculosis is a potent inducer of cytokine production by mononuclear phagocytes, which are an important cellular component in the first line immune defence. In this study, the cell wall-associated Triton X-100 soluble protein (TSP) antigens, TSP-H37Rv, TSP-H37Ra, TSP-K, and TSP-BCG, were isolated from M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. tuberculosis K-strain, and M. bovis BCG, respectively. The monocytes were isolated from the peripheral blood mononuclear cells of healthy individuals and were co-cultured with each TSP antigens and the secretory proteins of M. tuberculosis (PPD and 30-kDa antigen) to measure the production of cytokines; tumor necrosis factor (TNF)-a, interleukin (IL)-12, IL-8 and monocyte chemotactic protein-1 (MCP-1). The TSP-H37Rv antigen- stimulated monocytes showed higher level of TNF-a and IL-12 production compared to those of other TSP antigens and PPD. Especially, IL-12 production in response to the TSP-H37Rv antigen was significantly elevated in comparison with that of PPD-stimulated monocytes (TSP-H37Rv, 255.5+/-256.9 pg/ml; PPD, 55.7+/-55.4 pg/ml). However, the 30-kDa antigen did not induce TNF-alpha expression and also showed the lowest level of cytokine and chemokine production by monocytes. MCP-1 and IL-8 production were similarly increased in response to all TSP antigens and the PPD antigen. The production of IL-12 by the TSP-H37Rv antigen stimulation was significantly increased in PPD reactors than that in the non-reactor group, while the levels of other cytokines stimulated with each TSP antigens, 30-kDa and PPD antigen were not significantly different between the tuberculin reactor and the non-reactor groups. These results suggest that the cell wall-associated TSP antigen isolated from M. tuberculosis H37Rv acts as a more potent IL-12 inducer than the PPD antigen in innate immune response and thus it could further activate the Th1-mediated immune responses effectively against M. tuberculosis infection.
