Determination of dl-tetrahydropalmatine and imperatorin in Yuanhu Zhitong Prescription by RP-HPLC
- VernacularTitle:RP-HPLC测定元胡止痛方中延胡索乙素和欧前胡素的含量
- Author:
Yangyang ZHU
;
Boyang YU
- Publication Type:Journal Article
- Keywords:
Yuanhu Zhitong Prescription;
dl-tetrahydropalmatine;
imperatorin;
determination of concentration
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a RP-HPLC method for simultaneous determination of the dl-tetrahydropalmatine and imperatorin in Yuanhu Zhitong Prescription (Rhizoma Corydalis, Radix Angelicae Dahuricae). METHODS: dl- tetrahydropalmatine and imperatorin were extracted with ether and determined by HPLC using a BDS C 18 column(5?m,4.6?250mm),phosphate buffer(pH6.6)-methanol(39∶61) as a mobile phase. The mobile rate was at 1mL?min -1 and detection wavelength was at 280nm. RESULTS: The relationship between the concentration and the peak area of dl-tetrahydropamaltine and imperatorin were good linear relation, respectively. Either of correlation coefficient approached 0.999. CONCLUSION: This method can be used to determine the concentration of dl-tetrahydropalmatine and imperatorin in the preparation of Yuanhu Zhitong Prescription.
