Tissue culture of shoot-tip and plantlet regeneration of Camptotheca acumunata
- VernacularTitle:喜树茎尖组织培养与植株再生
- Author:
Litang LU
;
Dongxue ZHU
;
Degang ZHAO
- Publication Type:Journal Article
- Keywords:
Camptotheca acuminataDecne.;
plantlet regeneration;
tissue culture
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To explore the artificial propagation of the medicinal plant, Camptotheca acuminata Decne. Methods The shoot-tip was tested as the explants and cultured on culture media with different portions of hormone. Results The best medium for bud induction was the B5 basic medium with additions of 6-BA (0.2 mg/L), IBA (0.05 mg/L), and AS (afenine sulfate, 10 mg/L). While B5 with the additions of IBA (0.5 mg/L), KT (0.1 mg/L), and AS (10 mg/L) was suitable for rooting. The seedling was cultivated and grew well on the base material mixed with perlite-soil (3∶7). The survival rate of transplant was up to 96%. Conclusion The above mentioned method provides a new effective way to exploit this plant resources.
